Formation of 3-hydroxybutan-2-one and butane-2:3-diol by pig-heart preparations and its relationship to oxidation of pyruvate.
نویسندگان
چکیده
PAGE Inhibition of the alanine-oxoglutaric acid transaminase of pig-heart muscle by cyclic hydrazides.
منابع مشابه
Metabolism of acetoin in mammalian liver slices and extracts. Interconversion with butane-2,3-diol and biacetyl.
1. [(14)C]Acetoin was enzymically synthesized from [(14)C]pyruvate with a pyruvate decarboxylase preparation. Its optical activity was [alpha](20) (d)-78 degrees . 2. Large amounts (1000-fold higher than physiological concentrations) of acetoin were incubated with rat liver mince. Acetoin disappeared but very little (14)CO(2) was evolved. A compound accumulated, which was purified and identifie...
متن کاملRegulation of heart muscle pyruvate dehydrogenase kinase.
1. The activity of pig heart pyruvate dehydrogenase kinase was assayed by the incorporation of [(32)P]phosphate from [gamma-(32)P]ATP into the dehydrogenase complex. There was a very close correlation between this incorporation and the loss of pyruvate dehydrogenase activity with all preparations studied. 2. Nucleoside triphosphates other than ATP (at 100mum) and cyclic 3':5'-nucleotides (at 10...
متن کاملInhibition by pyruvate of pig heart mitochondrial glutamate influx.
Cardiac muscle mainly oxidises fatty acids, ketone bodies and pyruvate originating from either glycolysis or glucogenolysis, or from lactate oxidation. The formation of pyruvate and its oxidation by mitochondria are controlled directly by the reoxidation of extra-mitochondrial NADH, which cannot operate directly via the respiratory chain [ 1,2] . This reoxidation probably occurs via the Borst c...
متن کاملRegulation of gluconeogenesis and lipogenesis. The regulation of mitochondrial pyruvate metabolism in guinea-pig liver synthesizing precursors for gluconeogenesis.
1. The carboxylation of pyruvate to oxaloacetate by pyruvate carboxylase in guinea-pig liver mitochondria was determined by measuring the amount of (14)C from H(14)CO(3) (-) fixed into organic acids in the presence of pyruvate, ATP, Mg(2+) and P(i). The main products of pyruvate carboxylation were malate, fumarate and citrate. Pyruvate utilization, metabolite formation and incorporation of (14)...
متن کاملMETABOLIC STUDlES ON HEART MITOCHONDRIA*
Relatively few investigations have been made on the oxidation of pyruvate by heart mitochondria. Guinea pig heart mitochondria (1) and rabbit heart cyclophorase preparations (2, 3) have been found to oxidize pyruvate, but in no case has a detailed investigation been made of the mechanisms involved and the requirements for the operation of the complete tricarboxylic acid cycle. On the other hand...
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ورودعنوان ژورنال:
- The Biochemical journal
دوره 67 3 شماره
صفحات -
تاریخ انتشار 1957